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Mammaglobin 1 antibody Anti-Mammaglobin 1|ELISA WB

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Criterion™ Blotter | Life Science Research | Bio-Rad

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Neutrophil Elastase Inhibitor Assay Kit (ab118971) | Abcam

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Clean-Blot™ IP Detection Reagent (HRP)

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and ...

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Anti-mouse IgG, HRP-linked Antibody | Cell Signaling ...

Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Limited Uses. Except as otherwise ...

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Inhibition of the de novo pyrimidine biosynthesis pathway ...

Western blot analysis indicated that DHODH and UMPS protein levels were higher in the glioblastoma cells LN229, ... Eur J Biochem. 2000;267(7):2079–87. Epub 2000/03/23. pmid:10727948. View Article PubMed/NCBI Google Scholar 5. Fang J, Uchiumi T, Yagi M, Matsumoto S, Amamoto R, Takazaki S, et al. Dihydro-orotate dehydrogenase is physically associated with the respiratory complex and its loss ...

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Increased processing of SINE B2 ncRNAs unveils a novel ...

After incubating primary antibody, blots were washed using 0.1% PBST and reprobed with anti-rabbit-HRP conjugate secondary antibody (1:5000; abcam; ab97051). Proteins were visualized using Pierce enhanced chemiluminescence detection system (Thermo Fisher Scientific; 32106). Blots were imaged in an AI600 imager (GE Healthcare) and densitometry ...

Expression of a Bacillus Phytase C Gene in Pichia pastoris ...

Enzyme kinetics were determined for PhyC-R. Phytase activity was tested using sodium phytate (ranging from 0.03 to 1.60 mM) in 100 mM Tris-HCl buffer (pH 7.5) with 1 mM CaCl 2. Thermal stability was evaluated by measuring phytase activity after incubation at 37, 60, or 80°C for 10 min in 100 mM Tris-HCl (pH 7.5) or in 360 mM sodium acetate (pH 5.5) in the presence of 1 or 5 mM CaCl 2 .

MAB2079Z | Sigma-Aldrich

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Effector prediction and characterization in the oomycete ...

Author summary There is a molecular battle that takes place during infection of plants and animals by pathogens. Some of the weapons in the pathogen’s arsenal are known as “effectors;” these are secreted proteins that enter host cells to alter their physiology and suppress the immune system. Effectors can also be a liability for pathogens because potential hosts have evolved ways to ...

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DNA Nanomachines for Identifying Cancer Biomarkers in Body ...

Identifying molecular biomarkers promises to significantly improve the accuracy in cancer diagnosis at its early stage. DNA nanomachines, which are designable and switchable nanostructures made of DNA, show broad potential to detect tumor biomarkers with noninvasive, inexpensive, highly sensitive, and highly specific advantages. This Feature summarizes the recent DNA nanomachine-based ...

MLLT6 maintains PD‐L1 expression and mediates tumor immune ...

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Anti-Meningococcal human reference serum CDC1992

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53467 | Sigma-Aldrich

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RTEL1 Regulates G4/R-Loops to Avert Replication ...

Rtel1 Deletion and G4 Stabilization Change Transcriptional Landscape in a Similar Pattern. To investigate the source of replication aberrations in Rtel1-deficient cells, we used an established Rtel1 F/F Cre-lox system to conditionally inactivate Rtel1 in mouse embryonic fibroblasts (MEFs). Infection of cells with a Cre-GFP-expressing adenovirus, but not a GFP-expressing control, results in ...

Immunoprecipitation / Western Blots - SCBT

Immunoprecipitation / Western Blots. Prepare a total cell lysate as described under Western blot procedure in protocol 1. Preclear whole cell lysate (optional step) as follows: To approximately 1 ml of whole cell lysate or tissue extract (see Whole Cell Lysates or Tissue Extract Tables), add 0.25 µg of the appropriate control IgG (corresponding to the host species of the primary antibody ...

Alterations in Glucose Metabolism Due to Decreased ...

(Merck Millipore, MA, USA, 03-100 at 1:10,000) or mouse anti- -actin antibody (Santa Cruz Biotechnology, Inc., Dallas, ... BloT Quant HRP or Ultra Sensitive HRP Substrate (Takara Bio, Inc., Shiga, Japan) and Amersham hyperfilm ECL (GE Healthcare Ltd., Buckinghamshire, UK). 2.6. Cell Proliferation Assay After the cells were cultured for 24 to 60 h, a volume of 10 L/well of the Cell Counting ...

Bedrock radioactivity influences the rate and spectrum of ...

Fourteen species with contrasted bedrock radioactivity exposure were selected (black dot: low exposure, red dot: high exposure). Based on their phylogenetic history (a), we further selected six monophyletic pairs of closely related species to compare their mutational spectrum (Vielvic and Montbar are excluded because of unresolved phylogeny, pairs are indicated using superscript numbers).

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Biology | Free Full-Text | Alterations in Glucose ...

After washing with PBS containing 0.05% Tween 20 (PBS-T), the membranes were incubated with a mouse anti-HNRNPM antibody (Merck Millipore, MA, USA, 03-100 at 1:10,000) or mouse anti-β-actin antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, sc-47778 at 1:12,000) diluted in Can Get Signal ® Solution 1 (Toyobo

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Northern Blot Analysis. Total RNA of about 10 μg was treated with DNase I and separated on a 1.2% formaldehyde-deformed agarose gel. RNA was transferred to nitrocellulose membranes and fixed for 30 min at 120°C. DIG Oligonucleotide Tailing Kit was used for digoxigenin tailing. DIG-probes (10 pM) were used for hybridization at 50°C overnight ...

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Western Blot Analysis of Fas and FasL. Proteins were extracted from cells with the use of Trizol reagent (Life Technologies, Inc.) according to the manufacturer's instructions. The extract was analyzed for protein concentration with a Lowry protein assay kit (Bio-Rad Laboratories, Hercules, CA). Equal amounts of protein (25 μg) from each ...

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Myc-Tag (71D10) Rabbit mAb | Cell Signaling Technology

Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right). Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (left) versus wild-type 293 cells (right) using Myc-Tag (71D10) Rabbit mAb (green). Blue = DAPI #4083 ...

MicroRNA induction by copy number gain is associated with ...

Introduction. Copy number alternations, such as genomic amplifications and deletions, induce oncogene expression and repress tumor suppressor genes 1.Even low-level copy number gains have been found to contribute to oncogene induction and have been associated with malignant phenotypes.

Transcription-Replication Conflict Orientation Modulates R ...

(C) Southern blot of EcoRI digested DNA samples from mAIRN HO or mAIRN CD cells after induction of transcription with 0, 10, 50, 100, 500 or 1000 ng/mL DOX for 72h. Black triangles and red bars indicate positions of EcoRI restriction sites and the mAIRN probe used to visualize a 6.8kb or 3.2kb fragment of the mAIRN HO or CD construct, respectively. The blot was subsequently stripped and probed ...

PNOCARC Neurons Promote Hyperphagia and Obesity upon High ...

Calorie-rich diets induce hyperphagia and promote obesity. Here, Jais et al. report that short-term high-fat-diet (HFD) feeding in mice activates prepronociceptin (PNOC)-expressing neurons in the arcuate nucleus of the hypothalamus (ARC). They characterize PNOCARC neurons as a novel ARC neuron population activated upon palatable food consumption to promote hyperphagia.

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Expression of EPGN (ALGV3072, EPG, epigen, PRO9904) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.

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BAZ1B in Nucleus Accumbens Regulates Reward-Related ...

ATP-dependent chromatin remodeling proteins are being implicated increasingly in the regulation of complex behaviors, including models of several psychiatric disorders. Here, we demonstrate that Baz1b , an accessory subunit of the ISWI family of chromatin remodeling complexes, is upregulated in the nucleus accumbens (NAc), a key brain reward region, in both chronic cocaine-treated mice and ...

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